HPLC法同時測定3種紅景天藥材中5種化學(xué)成分的含量
發(fā)布時間:2019-08-30 來源: 日記大全 點(diǎn)擊:
中圖分類號 R284 文獻(xiàn)標(biāo)志碼 A 文章編號 1001-0408(2018)18-2515-05
DOI 10.6039/j.issn.1001-0408.2018.18.16
摘 要 目的:建立同時測定大花紅景天、狹葉紅景天和長鞭紅景天藥材中沒食子酸、紅景天苷、酪醇、咖啡酸、對香豆酸含量的方法,并比較3種紅景天藥材中5種化學(xué)成分的含量差異。方法:采用高效液相色譜法測定含量,色譜柱為WondaSil C18,流動相為乙腈-0.3%磷酸溶液(梯度洗脫),檢測波長為275 nm,柱溫為25 ℃,流速為1.0 mL/min,進(jìn)樣量為10 μL;采用GraphPad Prism 5.0軟件對含量測定結(jié)果進(jìn)行單因素方差分析和Tukey’s多重比較。結(jié)果:沒食子酸、紅景天苷、酪醇、咖啡酸、對香豆酸的檢測進(jìn)樣量線性范圍分別為0.020 48~0.614 4 ?g(r=0.999 7)、0.118~3.54 ?g(r=0.999 5)、0.010 84~0.325 2 ?g(r=0.999 7)、0.008 48~0.254 4 ?g(r=0.999 5)、0.004 1~0.123 ?g(r=0.999 5);精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD均小于2%;加樣回收率分別為99.62%~104.92%(RSD=1.68%,n=9)、96.55%~100.69%(RSD=1.67%,n=9)、98.91%~103.39%(RSD=1.48%,n=9)、100.93%~104.98%(RSD=1.37%,n=9)、97.71%~103.89%(RSD=1.99%,n=9)。大花紅景天中沒食子酸、紅景天苷、酪醇和咖啡酸含量均顯著高于長鞭紅景天,大花紅景天中的紅景天苷、咖啡酸含量均顯著高于狹葉紅景天,差異均有統(tǒng)計學(xué)意義(P<0.05,P<0.01或P<0.001)。結(jié)論:該方法簡便可行、結(jié)果準(zhǔn)確,可用于同時測定大花紅景天、狹葉紅景天和長鞭紅景天藥材中沒食子酸、紅景天苷、酪醇、咖啡酸、對香豆酸的含量。3種常見紅景天藥材中的化學(xué)成分含量存在較大差異,大花紅景天中紅景天苷的含量最高。
關(guān)鍵詞 高效液相色譜法;單因素方差分析;Tukey’s多重比較;紅景天;沒食子酸;紅景天苷;酪醇;咖啡酸;對香豆酸;含量
ABSTRACT OBJECTIVE: To establish a method for simultaneous determination of gallic acid, salidroside, tyrosol, caffeic acid and p-coumaric acid in Rhodiola crenulata, R. kirilowii and R. fastigiata, and to compare the content difference of 5 chemical components in 3 kinds of R. rosea. METHODS: HPLC method was adopted. The separation was performed on WondaSil C18 column with mobile phase consisted of acetonitrile-0.3% phosphoric acid solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 275 nm, and the column temperature was 25 ℃. Sample size was 10 μL. GraphPad Prism 5.0 software was used for single factor variance analysis and Tukey’s multiple comparison of content determination results. RESULTS: The linear ranges of gallic acid, salidroside, tyrosol, caffeic acid and p-coumaric acid were 0.020 48-0.614 4 ?g(r=0.999 7), 0.118-3.54 ?g(r=0.999 5), 0.010 84-0.325 2 ?g(r=0.999 7), 0.008 48-0.254 4 ?g(r=0.999 5) and 0.004 1-0.123 ?g(r=0.999 5), respectively. RSDs of precision, stability and reproducibility tests were all lower than 2%. The recoveries were 99.62%-104.92%(RSD=1.68%,n=9), 96.55%-100.69%(RSD=1.67%,n=9), 98.91%-103.39%(RSD=1.48%,n=9), 100.93%-104.98%(RSD=1.37%,n=9), 97.71%-103.89%(RSD=1.99%,n=9), respectively. The contents of gallic acid, salidroside, tyrosol and caffeic acid in R. crenulata were higher than R. fastigiata; the contents of salidroside and caffeic acid in R. crenulata were significantly higher than R. kirilowii, with statistical significance (P<0.05,P<0.01 or P<0.001). CONCLUSIONS: The method is simple, feasible and accurate, and can be used for simultaneous determination of gallic acid, salidroside, tyrosol, caffeic acid and p-coumaric acid in R. crenulata, R. kirilowii and R. fastigiata. There was great difference in the contents of chemical components in 3 kinds of common R. rosea, the content of salidroside is the highest in R. crenulata.
相關(guān)熱詞搜索:測定 藥材 含量 化學(xué)成分 紅景天
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