www.黄片视频在线播放,欧美精品日韩精品一级黄,成年男女免费视频网站,99久久久国产精品免费牛牛四川,99久久精品国产9999高清,乱人妻中文字幕视频4399,亚洲男人在线视频观看

超聲波輔助提取—?dú)庀嗌V法測(cè)定茶葉中9種有機(jī)磷農(nóng)藥殘留

發(fā)布時(shí)間:2019-09-02 來(lái)源: 美文摘抄 點(diǎn)擊:

http://img1.qikan.com.cn/qkimages/hbny/hbny201621/hbny20162148-1-l.jpghttp://img1.qikan.com.cn/qkimages/hbny/hbny201621/hbny20162148-2-l.jpghttp://img1.qikan.com.cn/qkimages/hbny/hbny201621/hbny20162148-3-l.jpghttp://img1.qikan.com.cn/qkimages/hbny/hbny201621/hbny20162148-4-l.jpghttp://img1.qikan.com.cn/qkimages/hbny/hbny201621/hbny20162148-5-l.jpghttp://img1.qikan.com.cn/qkimages/hbny/hbny201621/hbny20162148-6-l.jpghttp://img1.qikan.com.cn/qkimages/hbny/hbny201621/hbny20162148-7-l.jpg
  摘要:建立了一種超聲波輔助提取-氣相色譜法測(cè)定茶葉中9種有機(jī)磷農(nóng)藥殘留的檢測(cè)方法。茶葉磨碎,丙酮-正己烷(體積比為1∶1)超聲波提取,活性炭小柱凈化,丙酮定容,氣相色譜-火焰光度法(GC-FPD)進(jìn)行檢測(cè),外標(biāo)法定量。以9種有機(jī)磷農(nóng)藥(敵敵畏、甲胺磷、乙酰甲胺磷、樂(lè)果、毒死蜱、殺螟硫磷、喹硫磷、三唑磷、水胺硫磷)為分析物,考察并優(yōu)化了色譜條件、提取溶劑、凈化條件和基質(zhì)效應(yīng),確定了最佳試驗(yàn)條件。在最優(yōu)試驗(yàn)條件下,回歸方程線性范圍為0.020~0.120 μg/mL,R2>0.99,方法檢出限為0.004 0~0.010 0 mg/kg;9種有機(jī)磷農(nóng)藥殘留混標(biāo)于0.025、0.040和0.080 μg/mL 3個(gè)加標(biāo)水平的回收率范圍為80.88%~125.46%,相對(duì)標(biāo)準(zhǔn)偏差(RSD,n=6)為1.36%~10.75%。該方法準(zhǔn)確、穩(wěn)定,可滿足茶葉中9種有機(jī)磷農(nóng)藥殘留的檢測(cè)要求。
  關(guān)鍵詞:超聲波輔助提。粴庀嗌V法;茶葉;有機(jī)磷農(nóng)藥殘留
  中圖分類號(hào):O657.6;S482 文獻(xiàn)標(biāo)識(shí)碼:A 文章編號(hào):0439-8114(2016)21-5623-05
  DOI:10.14088/j.cnki.issn0439-8114.2016.21.048
  Determination of 9 Organophosphorus Pesticide Residues from Tea by Ultrasonic-assisted Extraction and Gas Chromatography
  CHEN Xiao-quan, YU Xing, WU Xiao-gang, XIAO Hai-jun, LIU Bin-qiu,
  XU Yi-yin, LING Deng-meng, SANG Shou-qiang, LIANG Jun-tao
  (Lab of Technology Center, Menghai Tea Industry Co., Ltd in TAETEA Group, Menghai 666200, Yunnan, China)
  Abstract:A gas chromatographic method for the simultaneous determination of 9 organophosphorus pesticides residues in tea was established. Samples were ground,extracted with acetone-hexane(1∶1,V/V),then was cleaned-up with a activated carbon cartridge,dissolved in acetone,analyzed by GC-FPD and quantified by external standard method. With 9 organophosphorus pesticides including dichlorovos,methamidophos,acephate,dimethoate,chlorpyrifos,fenitrothion,quinalphos,triazophos and isocarbophos as analytes,the chromatographic conditions,extraction solvents,purification conditions and matrix effect were investigated and optimized to determine the optimal experimental conditions. Under the optimal experimental conditions,the method showed a good linearity(R2>0.99) in the range of 0.020~0.120 μg/mL,the limit of detections were 0.004 0~0.01 mg/kg. At the spiked levels of 0.025,0.040 and 0.080 μg/mL,the recoveries of 9 analytes were in the range of 80.88%~125.46%,and the relative standard deviations(RSDs,n=6) were 1.36%~10.75%. The method was accurate,stable,and could meet requirements for the detection of 9 organophosphorus pesticides from tea.
  Key words:ultrasonic-assisted extraction;gas chromatography(GC);tea;organophosphorus pesticide residues
  茶葉是世界各國(guó)人們喜愛(ài)的飲品,也是中國(guó)主要的出口經(jīng)濟(jì)作物產(chǎn)品之一,其農(nóng)藥殘留問(wèn)題一直受到廣泛關(guān)注[1]。茶葉種植中,有機(jī)磷農(nóng)藥因可用于防治蟲(chóng)害而成為最常噴施的農(nóng)藥種類,其半衰期相對(duì)較長(zhǎng)[2],對(duì)飲茶者的健康造成潛在危害。茶葉進(jìn)口囯歐盟、日本等地對(duì)進(jìn)口茶葉中的有機(jī)磷農(nóng)藥殘留制定了苛刻的限量標(biāo)準(zhǔn),中國(guó)相關(guān)部門(mén)也發(fā)布多個(gè)茶葉有機(jī)磷農(nóng)藥殘留限量標(biāo)準(zhǔn)[3,4]。
  國(guó)內(nèi)外關(guān)于對(duì)有機(jī)磷農(nóng)藥殘留的檢測(cè)已有大量報(bào)道,常用的檢測(cè)方法有氣相色譜法[5-7]、氣相色譜質(zhì)譜聯(lián)用法[8,9]、液相色譜串聯(lián)質(zhì)譜法[10-13]、固相微萃取-離子遷移率譜法[14]等。茶葉中的有機(jī)磷含量較低且茶葉內(nèi)含物質(zhì)成分豐富、基質(zhì)復(fù)雜,干擾組分多,現(xiàn)階段多數(shù)實(shí)驗(yàn)室對(duì)茶葉中有機(jī)磷農(nóng)藥殘留的檢測(cè)較為困難。探索茶葉中有機(jī)磷農(nóng)藥殘留檢測(cè)方法具有重大意義。

相關(guān)熱詞搜索:色譜 超聲波 農(nóng)藥 殘留 提取

版權(quán)所有 蒲公英文摘 www.91mayou.com