[摘要] 建立山茱萸藥材中環(huán)烯醚萜苷類及三萜酸類化學(xué)成分的含量測(cè)定方法，為山茱萸藥材的質(zhì)量標(biāo)準(zhǔn)提升提供技術(shù)支撐。采用HPLC-UV法，以乙腈-0.1%甲酸水溶液為流動(dòng)相梯度洗脫，檢測(cè)波長(zhǎng)為240 nm，建立同時(shí)測(cè)定莫諾苷、馬錢(qián)苷、當(dāng)藥苷的含量測(cè)定方法;采用HPLC-ELSD法，以甲醇-0.5%乙酸銨（87∶13）為流動(dòng)相，建立同時(shí)測(cè)定齊墩果酸、熊果酸的含量測(cè)定方法。結(jié)果顯示在上述色譜條件下，莫諾苷、馬錢(qián)苷、當(dāng)藥苷分別在質(zhì)量濃度5.335～213.4 mg·L^-1（r = 0.999 9），5.515～220.6 mg·L^-1 （r=1.000 0），1.992～79.68 mg·L^-1（r=1.000 0）線性關(guān)系良好，平均加樣回收率均在98.49%～99.28%，RSD<2%;齊墩果酸、熊果酸分別在質(zhì)量濃度7.74～154.8 mg·L^-1（r=0.996 4），10.82～216.4 mg·L-1 （r=0.999 6）線性關(guān)系良好，平均加樣回收率均在98.11%～99.27%，RSD <3%。表明所建立的方法簡(jiǎn)單快速，準(zhǔn)確可靠，重復(fù)性好，可用于客觀評(píng)價(jià)山茱萸藥材的品質(zhì)及其質(zhì)量控制，為完善山茱萸藥材現(xiàn)行質(zhì)量標(biāo)準(zhǔn)提供了實(shí)驗(yàn)數(shù)據(jù)，對(duì)于保證其質(zhì)量和臨床療效具有重要意義。
　　[關(guān)鍵詞] 山茱萸;環(huán)烯醚萜苷;三萜酸;藥材質(zhì)量標(biāo)準(zhǔn)制定
　　[收稿日期] 2015-04-12
　　[基金項(xiàng)目] 國(guó)家自然科學(xué)基金項(xiàng)目（81473538）;教育部霍英東教育基金會(huì)高等院校青年教師基金項(xiàng)目（141040）;江蘇高校優(yōu)勢(shì)學(xué)科建設(shè)工程資助項(xiàng)目（ysxk-2014）
　　[通信作者] *段金廒，教授，博士研究生導(dǎo)師，Tel： （025）85811116，E-mail： dja@njucm.edu.cn
　　[作者簡(jiǎn)介] 郭盛，博士，主要從事中藥資源化學(xué)研究及教學(xué)工作，Tel：（025）85811916，E-mail：guosheng@njucm.edu.cn
　　Establishment of quality standard for Corni Fructus based on
　　the multiple bioactive constituents
　　GUO Sheng，  DUAN Jin-ao*，  ZHU Shao-qing，  QIAN Da-wei
　�。↗iangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， and National and
　　Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative
　　Medicine， Nanjing University of Chinese Medicine， Nanjing 210023， China）
　　[Abstract] This study is to establish the methods for determination of iridoid glycosides and triterpenic acids in Corni Fructus and provide technical support for the quality control of Corni Fructus.Morroniside， loganin and sweroside were determined by HPLC-UV method with acetonitrile and 0.1% formic acid as the mobile phase， and the detective wavelength was set at 240 nm. Oleanolic acid and ursolic acid were determined by HPLC-ELSD method with methanol-0.5% ammonium acetate （87∶13） as the mobile phase. The results showed that  the linear ranges of morroniside， loganin and sweroside were 5.335-213.4 mg·L^-1 （r=0.999 9）， 5.515-220.6 mg·L^-1（r=1.000 0）， 1.992-79.68 mg·L^-1（r=1.000 0）， respectively. The average recoveries of the above three iridoid glycosides were 98.49%-99.28% with RSDs of recoveries being less than 2%.The linear ranges of oleanolic acid and ursolic acid were 7.74-154.8 mg·L-1 （r=0.996 4），10.82-216.4 mg·L-1 （r=0.999 6）， respectively. The average recoveries of the above two triterpenic acids were 98.11%-99.27% with RSDs of recoveries being less than 3%. The method established in this research is simple， rapid and reliable， and can be used for quality control of Corni Fructus. Furthermore， the research provided experimental data for the improvement of present quality standard of Corni Fructus， which has important significance to guarantee its quality and clinical curative effect.

相關(guān)熱詞搜索：山茱萸 藥材 質(zhì)量標(biāo)準(zhǔn) 功效 成分